2024 Phenylacetate-coa

Phenylacetate-coa

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WebAug 10, 2010 · Although a phenylacetate metabolic gene cluster had been identified, the underlying biochemistry remained largely unknown. Here we elucidate the catabolic pathway functioning in 16% of all... WebThe aerobic strategy is illustrated by the metabolism of phenyl-acetate and phenylacetyl-CoA, which are derived froma varietyof substrates such as phenylalanine, lignin-related …

UniProt

WebPhenylacetate. Optimal Result: 0 - 0.18 mcg/mg creatinine. Interpret your laboratory results instantly with us. Phenylacetate is produced from bacterial degradation of unabsorbed … ghostbusters sigourney weaver

CDD Conserved Protein Domain Family: PA_CoA_ligase

WebPhenylacetate is derived from the metabolism of phenylalanine, Z-phenylacetaldoxime, styrene, and many other compounds. Phenylacetate aerobic metabolism in fungi usually proceeds by oxidation to homogentisate ( Cox et al., 1996 ), as shown in the left branch below. In bacteria, this rarely occurs, rather the initial step is ligation with CoA. WebCatalyzes the activation of phenylacetic acid (PA) to phenylacetyl-CoA (PA-CoA). Involved in the phenylalanine metabolism. 2 publications. Catalytic activity 2-phenylacetate + ATP + … WebDownload alignment. phenylacetate-CoA oxygenase, PaaG subunit. Phenylacetate-CoA oxygenase is comprised of a five gene complex responsible for the hydroxylation of phenylacetate-CoA (PA-CoA) as the second catabolic step in phenylacetic acid (PA) degradation. Although the exact function of this enzyme has not been determined, it has … ghostbusters simulator

National Center for Biotechnology Information

UniProt

WebMar 10, 2015 · The phenylacetate-CoA binds to the PaaA subunit in a hairpin conformation within a ~20Å deep pocket in the protein. The adenine and phenyl moieties at the two ends of the substrate point in the same direction and the phosphates are at the hairpin bend near the protein surface (Figure 2A). The entrance to the substrate-binding pocket is ... WebThe Azoarcus evansii gene which codes for phenylacetate-CoA ligase, an enzyme involved in the aerobic degradation of phenylacetate, was isolated from a genomic library, using as the probe a fragment of the gene which encodes the isoenzyme that is induced under anaerobic conditions. By this means both the gene and its flanking sequences were … front access rucksackWebPhenylacetate-CoA ligase (E.C. 6.2.1.30), the initial enzyme in the metabolism of phenylacetate, was studied in Thermus thermophilus strain HB27. Enzymatic activity … ghostbusters sigourney

"WebApr 21, 2006 · Phenylacetate derives primarily from phenylalanine, but it is also formed in degradation pathways that target a variety of environmental aromatics of natural and synthetic origin and, thus, is the central element of an important catabolon ( 20, 21, 22 ). " - Phenylacetate-coa

Phenylacetate-coa

Phenylacetate Metabolism in Thermophiles: Characterization of

Web2-phenylacetate + ATP + CoA = AMP + diphosphate + phenylacetyl-CoA 1 publication This reaction proceeds in the forward direction. 1 publication EC:6.2.1.30 ( UniProtKB ENZYME Rhea) Source: Rhea 20956 Hide Rhea reaction 2-phenylacetate CHEBI:18401 zoom + ATP CHEBI:30616 zoom + CoA CHEBI:57287 zoom = AMP CHEBI:456215 zoom + diphosphate WebIn enzymology, a phenylacetate—CoA ligase is an enzyme (EC 6.2.1.30) that catalyzes the chemical reaction. ATP + phenylacetate + CoA AMP + diphosphate + phenylacetyl-CoA. …

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Webphenylacetic acid: [ fen″il-ah-se´tik ] a catabolite of phenylalanine ; it is excessively formed and excreted, sometimes conjugated with glutamine , in phenylketonuria . WebMethyl phenylacetate undergoes decomposition on photolysis in methanol. Methyl phenylacetate acts as acylating agent and causes the enantioselective acylation of beta …

WebApr 15, 2008 · The phenylacetate-CoA ligase gene ( paaK) was cloned and heterologously expressed in Escherichia coli and the recombinant protein was purified. The enzyme catalyzed phenylacetate + CoA + MgATP → phenylacetyl-CoA + AMP + MgPP i with a V max of 24 μmol/min/mg protein at a temperature optimum of 75°C. WebDec 23, 2008 · The phl gene, encoding a PCL (phenylacetate–CoA ligase), was cloned in Escherichia coli as a maltose-binding protein fusion and the biochemical properties of the enzyme were characterized. The recombinant fusion protein converted PAA into phenylacetyl-CoA in an ATP- and magnesium-dependent reaction.

WebPhenylalanine was completely oxidized and served as the sole source of cell carbon. Evidence is presented that degradation proceeds via benzoyl-CoA as the central aromatic intermediate; the aromatic ring-reducing enzyme benzoyl-CoA reductase was present in cells grown on phenylalanine. WebApr 15, 2008 · The phenylacetate-CoA ligase gene (paaK) was cloned and heterologously expressed in Escherichia coli and the recombinant protein was purified. The enzyme …

WebJul 21, 2010 · Phenylacetyl-CoA is the substrate of a presumed multicomponent oxygenase, PaaABCDE. This oxygenase is a key enzyme of the pathway, proposed to be responsible for the introduction of oxygen into the aromatic ring of phenylacetyl-CoA ( 6, 14, 21 ). First we … We would like to show you a description here but the site won’t allow us.

WebPhenylacetate-CoA oxygenase/reductase, PaaK subunit Imported. Gene names. Name. paaK Imported. ORF names. BW40_01826 Imported. Organism names. Organism. Micrococcus luteus (Micrococcus lysodeikticus) Imported. Taxonomic identifier. 1270 NCBI. Taxonomic lineage. Bacteria > Actinobacteria > Micrococcales > Micrococcaceae > … front accounting demoWebNational Center for Biotechnology Information front access pool skimmerWebNov 1, 2008 · The phl gene, encoding a PCL (phenylacetate–CoA ligase), was cloned in Escherichia coli as a maltose-binding protein fusion and the biochemical properties of the enzyme were characterized. The... front abs wire clips on a honda elementWebJun 1, 1993 · The enzyme catalysing the first step in the anaerobic degradation pathway of phenylacetate was purified from a denitrifying Pseudomonas strain KB 740. It catalyses the reaction phenylacetate+CoA+ATP → phenylacetyl-CoA+AMP+PPi and requires Mg2+. Phenylacetate-CoA ligase (AMP forming) was found in cells grown anaerobically with … front access skimmer for gunite poolWebPhenyl acetate is an aromatic ester. Phenyl acetate levels in urine are marker for the diagnosis of some forms of unipolar major depressive disorders. It undergoes Fries … front access cisternWebDec 23, 2008 · The phl gene, encoding a PCL (phenylacetate–CoA ligase), was cloned in Escherichia coli as a maltose-binding protein fusion and the biochemical properties of the … front abs sensor 2016 f250http://eawag-bbd.ethz.ch/pac/pac_map.html front accounting developers