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Immediate, multiplexed and sequential genome engineering …
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Metabolic engineering of - ScienceDirect
WebNov 2, 2024 · The crRNA expression cassettes systematically comprised the RNA polymerase III dependent SNR52 promoter, the target sequence (s) flanked by direct … WebJan 28, 2024 · SUP4TT. Multiple-gene cassette. insertion into multiple-gene. loci: 2.1%. Multiplex knock-in Delete KU80 gene to. increase the. frequency of HR. Horwitz et al., … WebJul 17, 2024 · The transformants were generated using four different combinations of constructs: donor only (Topo-ΔShXDR1), donor plus Cas9 (p426-SNR52p-gRNA.csr-1.Y-SUP4t) plus gRNA-XDR1 (p426-SNR52p-gRNA.ShXDR1.Y-SUP4t), split marker approach only (one with 1 kb of upstream region of ShXDR1 and 731 bp of hph and one with 1 kb of … clerk of court arapahoe county colorado